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Neurobehavioral and neuropathological studies of the protective effects of alpha lipoic acid (α-LA) against lead toxicity in rats

Eman Mohamed Abdella, Suzan F El-Sisy, Heba Ahmed El-Dash.

Abstract
Aim: The present study was conducted to elucidate the possible protective effect of alpha lipoic acid (α-LA) against the deleterious effect perturbation induced in rat brain exposed to lead acetate.
Methods: 32 Wistar male rats (weighing 130 ± 10 g) were divided into four groups (n=8): (1) normal control group (C); (2) Initiation group (Pb as lead acetate 20 mg/kg.b.wt, i.p. for 2 wks); (3) treatment group (α-LA 20 mg/kg.b.wt, i.p. for 3 wks); (4) post-initiation treatment group (Pb for 2 wks then followed by α-LA for 3 wks). Levels of monoamines (norepinephrine NE and dopamine DA), the level of Ache activity and finally adenosine triphosphate (ATP), were estimated in the hippocampus and cerebral cortex, in addition, a Morris water maze and the histological study were performed after completion of the experiments.
Results: The results of the present work demonstrated that Pb inhibited neurotransmitters releases and decrease the level of Ache activity, as well as it inhibited energy production ATP. Pb impaired performance on Morris Water Maze of rats and histological degeneration. However, treatment with α-LA significantly attenuated the behavioral impairment and biochemical parameters in rat treated with Pb. And amelioration of histological changes.
Conclusion: As a conclusion, treatment with α-LA can improve the Pb-induced toxicity via antioxidant activity.

Key words: Neurotoxicity; Alpha Lipoic Acid; Histopathology; Lead Toxicity; Animal behavior



Kurşun zehirlenmesine karşı alfa lipoik asitin (α-LA) koruyucu etkisinin nörodavranışsal ve nöropatolojik olarak değerlendirilmesi

Özet
Amaç: Çalışmanın amacı, rat beyninde kurşun asetata maruziyet sonucu oluşan hasarın düzeltilmesinde alfa lipoik asitin (α-LA) koruyucu etkinliğinin araştırılmasıdır.
Yöntemler: 32 Wistar cinsi erkek rat (ağırlıkları 130 ± 10 g) her bir grupta 8 rat olacak şekilde 4 gruba ayrıldı: (1) Normal kontrol grubu (C); (2) 2 hafta boyunca i.p. 20 mg/kg kurşun (Pb) asetat verilen grup; (3) 3 hafta boyunca i.p. 20 mg/kg α-LA verilen grup; (4) 2 hafta 20 mg/kg kurşun verildikten sonra 3 hafta boyunca α-LA verilen grup. Adenozin trifosfat (ATP) ile monoaminlerin (norepinefrin, dopamin) miktarı ve asetilkolin esteraz (Ache) aktivitesi hipokampüs ve serebral kortekste tespit edildi. İlave olarak ratlara Morris su labirenti testi uygulandı ve elde edilen beyin dokularından histopatolojik inceleme yapıldı.
Bulgular: Elde edilen bulgular kurşunun nörotransmitter salınımını bloke ettiğini, Ache aktivitesini azalttığını ve enerji üretimini inhibe ettiğini göstermektedir. Kurşun aynı zamanda ratların Morris su labirenti test performansını düşürdü ve histopatolojik dejenerasyona neden oldu. α-LA verildiğinde ise kurşun tarafından bozulan biyokimyasal parametreler, histopatolojik değişiklikler ve davranışsal bozukluklarda iyileşme izlendi.
Sonuç: α-LA antioksidan özelliğinden dolayı Pb tarafından indüklenen toksisitede düzelmeye neden olabilir.

Anahtar Kelimeler: Nörotoksisite; Alfa lipoik asit; Histopatoloji; Kurşun toksisitesi; Hayvan davranışı.


 
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How to Cite this Article
Pubmed Style

Abdella EM, El-Sisy SF, El-Dash HA. Neurobehavioral and neuropathological studies of the protective effects of alpha lipoic acid (α-LA) against lead toxicity in rats. doi:10.5455/spatula.20160504021625


Web Style

Abdella EM, El-Sisy SF, El-Dash HA. Neurobehavioral and neuropathological studies of the protective effects of alpha lipoic acid (α-LA) against lead toxicity in rats. http://www.spatuladd.com/?mno=222956 [Access: December 15, 2017]. doi:10.5455/spatula.20160504021625


AMA (American Medical Association) Style

Abdella EM, El-Sisy SF, El-Dash HA. Neurobehavioral and neuropathological studies of the protective effects of alpha lipoic acid (α-LA) against lead toxicity in rats. doi:10.5455/spatula.20160504021625



Vancouver/ICMJE Style

Abdella EM, El-Sisy SF, El-Dash HA. Neurobehavioral and neuropathological studies of the protective effects of alpha lipoic acid (α-LA) against lead toxicity in rats. doi:10.5455/spatula.20160504021625



Harvard Style

Abdella, E. M., El-Sisy, S. F. & El-Dash, H. A. (2017) Neurobehavioral and neuropathological studies of the protective effects of alpha lipoic acid (α-LA) against lead toxicity in rats. doi:10.5455/spatula.20160504021625



Turabian Style

Abdella, Eman Mohamed, Suzan F El-Sisy, and Heba Ahmed El-Dash. 2017. Neurobehavioral and neuropathological studies of the protective effects of alpha lipoic acid (α-LA) against lead toxicity in rats. doi:10.5455/spatula.20160504021625



Chicago Style

Abdella, Eman Mohamed, Suzan F El-Sisy, and Heba Ahmed El-Dash. "Neurobehavioral and neuropathological studies of the protective effects of alpha lipoic acid (α-LA) against lead toxicity in rats." doi:10.5455/spatula.20160504021625



MLA (The Modern Language Association) Style

Abdella, Eman Mohamed, Suzan F El-Sisy, and Heba Ahmed El-Dash. "Neurobehavioral and neuropathological studies of the protective effects of alpha lipoic acid (α-LA) against lead toxicity in rats." doi:10.5455/spatula.20160504021625



APA (American Psychological Association) Style

Abdella, E. M., El-Sisy, S. F. & El-Dash, H. A. (2017) Neurobehavioral and neuropathological studies of the protective effects of alpha lipoic acid (α-LA) against lead toxicity in rats. doi:10.5455/spatula.20160504021625